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ORIGINAL ARTICLE
Year : 2006  |  Volume : 54  |  Issue : 4  |  Page : 402-407

Brain edema after intracerebral hemorrhage in rats: The role of inflammation


1 Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China
2 Department of Neurology, The Beijing Shijitan Hospital, China
3 The Beijing Shijinshan Hospital, China

Correspondence Address:
Chunyan Li
The Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0028-3886.28115

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 ╗ Abstract 

Background: Intracerebral hemorrhage (ICH) results in secondary brain edema and injury that may lead to death and disability. ICH also causes inflammation. It is unclear whether inflammation contributes to brain edema and neuron injury or functions in repairing the brain tissue. Aims: To understand the effect of inflammation in ICH, we have carried out an investigation on the various aspects and the dynamic changes of inflammation. Settings and Design: An ICH model was generated by injecting 50 ml autologous tail artery blood stereotactically into the right caudate nucleus of 30 rats, which were randomly divided into five ICH groups. Similarly, five Sham control groups were generated by inserting the needle to the right caudate nucleus of rats. Materials and Methods: Rat behavior was evaluated over the time course (6 h, 24 h, 48 h, 72 h and 7 d) in each group. The rats were then killed by administering an overdose of pentobarbital. Following the euthanasia, the brain water content, neuronal loss, glia proliferation, inflammatory infiltration and brain morphology of the rats were measured. Additionally, the expression of TNF-a,IL-6, ICAM-1, VEGF, NF-kB, C3 and CR2 was analyzed by immunohistochemistry. Statistical Analysis: The data were analyzed by student's t test. Results: Rat brain water content increased progressively over the time course and reached its peak at 48h followed ICH. The maximum of inflammatory infiltrate (especially neutrophils) and immunopositive cells of TNF-a, IL-6 and NF-kB, were at 48h. The expression of C3 and CR2 reached their peaks at 48-72h, while the expression ICAM-1 and VEGF were at maximum at 72h followed ICH. Conclusions: The results suggested that the inflammatory cytokines, complement system and VEGF may have a function in the development of the brain edema and neuron injury followed ICH.


Keywords: Animal models, brain edema, inflammation, intracerebral hemorrhage


How to cite this article:
Zhang X, Li H, Hu S, Zhang L, Liu C, Zhu C, Liu R, Li C. Brain edema after intracerebral hemorrhage in rats: The role of inflammation. Neurol India 2006;54:402-7

How to cite this URL:
Zhang X, Li H, Hu S, Zhang L, Liu C, Zhu C, Liu R, Li C. Brain edema after intracerebral hemorrhage in rats: The role of inflammation. Neurol India [serial online] 2006 [cited 2019 Jul 23];54:402-7. Available from: http://www.neurologyindia.com/text.asp?2006/54/4/402/28115


Intracerebral hemorrhage (ICH) is a common and one of the most devastating types of stroke. ICH accounts for 10-15% of all strokes, but it is responsible for approximately 50% of stroke-related deaths. It imparts some form of disability to 88% of its survivors.[1],[2] Secondary brain edema and brain damage may result in further deterioration in neurological function after ICH. Although the mechanisms of brain injury after ICH are not fully understood, several mechanisms appear to contribute to edema development. Inflammatory processes appear to be involved in cardiovascular and cerebrovascular disease. Previous reports showed that ICH was accompanied by inflammation. Whether the inflammation contributes to brain edema and neurological injury or plays a role in repairing the brain tissue is not known. In order to understand the role of inflammation after ICH and provide information for developing new therapeutic strategies, we carried out an investigation on the dynamic changes of inflammatory responses such as cytokines, complement and blood-brain barrier breakdown.


 ╗ Materials and Methods Top


ICH rat model

The Institutional Animal Care and use committee and the local experimental ethics committee have approved all experimental procedures. Sixty male Sprague-Dawley rats (13-15 weeks) were used. The rats were anesthetized with pentobarbital (40 mg/kg, IP) and positioned in a stereotaxic frame (SR-6N, Japan). A cranial burr hole (1 mm) was drilled on the right coronal suture 3.5 mm lateral to the midline. ICH was initiated in 30 rats stereotactically by infusing 50 ml autologous tail artery blood into the right caudate nucleus at 10 ml/min through a 26-gauge needle (coordinates: 0.2 mm anterior, 5.5 mm ventral and 3.5 mm lateral to bregma). Thirty rats served as sham controls with only a needle insertion. At the end the needle was removed, the burr hole was sealed with bone wax, the wound was sutured and the animal was placed in a warm box with free access to food and water. Physiological parameters were maintained in the normal range in this experiment.

Behavior tests

Four motor behavior tests were carried out on rats by observers blinded to the experimental group or Sham group at time points 6h, 24h, 48h, 72h and seven days postoperation. (1) Longa behavioral test,[3] measured the spontaneous contralateral circling and tumble and was graded from 0 (no circling) to 4 (unconsciousness); (2) Berderson behavioral test,[4] measured the palsy of the contralateral limbs and was graded from 0 (no palsy) to 3 (circling of the contralateral); (3) Beam walking test,[5] measured the ability to walk on an 80-cm-long, 2.5-cm-wide wood beam and was graded from 0 for a rat that readily traversed the beam to 5 for a rat that was unable to move or fell off the beam; (4) Footfault asymmetry test,[6] measured the ability to walk in a net (the areole was 2.3 x 2.3 cm).

Brain water content measurement

At time points 6h, 24h, 48h, 72h and seven days, after the neurobehavioral tests were done, each rat was killed by a pentobarbital overdose. The brain was quickly removed and placed on a cooled surface. The frontal pole (approximately 3 mm thick),[3],[7] the cerebellum and brainstem were removed. The cerebrum was coronally divided into three pieces by sectioning through the needle entry site and the midpoint of the posterior remnant, respectively. The first piece (2 mm thick) was cut ipsilateral and contralateral of ICH, the two sections were used for brain edema measurement. Each section was wrapped in preweighed aluminum foil and weighed to obtain the wet weight (WW), then dried for 72h in an oven at 110░C and weighed again to obtain the dry weight (DW). Brain water content was calculated as the percentage change using the following formula: (WW- DW)/WW x 100%.

Histological examination

The other two coronal specimens from the cerebrum were used for histological examination. Briefly, they were perfused with 4% paraformaldehyde in 0.1mol/L PBS (pH 7.4) for three days and then dehydrated and embedded in paraffin. Sections (5 mm) were stained with hematoxylin and eosin or stained by routine immunohistochemical methods. Primary antibodies used were TNF-a (diluted 1:100, Santa Cruz, USA), IL-6 (diluted 1:50, Boshide company, China), ICAM-1 (diluted 1:100, Zhongshan biology technology company, China), VEGF (diluted 1:150, Zhongshan biology technology company, China), NF-kB (diluted 1:200, Santa Cruz, USA), C3 (diluted 1:500, Sigma, USA) and CR2 (complement receptor type 2, CD21) (diluted 1:500, Santa Cruz, USA). The secondary antibodies, secondary biotinylated conjugates and diaminobenzidine were from the Vector ABC kit (Zhongshan biology technology company, China). The immunohistochemical labeling and detection were performed following the protocols recommended by the manufacturers.

Statistical analysis

All the values were shown as mean and standard deviation (means ▒ SD), statistical analysis software SPSS 11.0 was used for data analysis. ANOVA and q tests were carried out for water content data and student's t -test was carried out for the rest of the data. Differences were considered significant at P <0.05.


 ╗ Results Top


High neurological deficit scores at 24h, 48h and 72h after operation

The behavior tests showed that the maximum neurological deficit scores in both ICH and Sham groups were at 6h postoperation, a phenomenon caused by the anesthesia and injuries during the operation [Figure - 1].1-1.4. The neurological deficit score of the ICH group maintained a high level at 24h, 48h and 72h, but decreased to the lowest level at seven days when the rats had recovered from the operation. In contrast, the scores of Sham control rats were lower than that of the ICH rats at 24h, 48h and 72h. The differences of the four behavior tests between the two groups were statistically significant, the P values at 24h, 48h, 72h were 0.011, 0.008, 0.002 respectively for the Longa test [Figure - 1] 1.1, 0.003, 0.012 and 0.001 for the Berderson test [Figure - 1]1.2, 0.02, 0.02 and 0.002 for the Beam Walking test [Figure - 1]1.3, and 0.041, 0.021, 0.004 for the Footfault asymmetry test [Figure - 1]1.4.

Significant difference of brain water content between ICH and Sham rats at 48h, 72h

Brain water content of the ICH and Sham groups showed no significant difference at 6h ( P =0.157) and 24h ( P =0.830) [Figure - 2]. At 48h and 72h, the brain water content of both ICH ipsilateral and contralateral samples was significantly higher than those of Sham group ( P< 0.001), with maximum water content observed at 48h. Significant differences were also observed between the ipsilateral and contralateral samples at 48h ( P = 0.0430) and 72h ( P = 0.049).

Significant changes in the brain tissue of rats in ICH group

A spherical hematoma was observed in the caudate nucleus area at all time points after ICH. At time point 6h, a few rounded scattered neutrophils were found around the periphery of the hematoma. No morphological changes of neurons were observed. At 24h, brain edema around the hematoma was visible, the infiltrated inflammatory cells were mainly mononuclear and several neutrophils could be seen. At 48h, the brain edema around the hematoma was pronounced and the brain tissue was diffluent and necrotic [Figure - 3]. The hematoma was surrounded by a compact band of cells including viable neutrophils, some cell debris, a few macrophages and rare clusters of intact erythrocytes. Degenerated neurons with vague nucleus and disappearing Nissl bodies and neuronophagia were also observed. At 72h, hematoma was surrounded by scattered glia cell containing hemosiderin, hyperplasia of glia and neovascularization. At time point seven days, the hematoma was resolving. The glia cell hyperplasia and neovascularization were abundant. Neutrophils were not present. In contrast, no hematoma or significant changes were observed in the brain tissue of rats from Sham group.

Expression of inflammatory factors

Positively stained neurons with a nucleus and normal morphology were counted as activated cells in three nonoverlapping regions under the microscope for the ipsilateral perihemotoma brain tissues. The expression of TNF-a [Figure - 4], ICAM-1 [Figure - 5], IL-6 [Figure - 6] and NF-kB [Figure - 7] after ICH were mainly in the cytoplasm of neurons and glia and reached their maximum at 48h or 72h. The expression of complement 3 [Figure - 8] and CR2 [Figure - 9] after ICH was up-regulated on the neurons, glia and endothelial cells in the perihemotoma. The expression of VEGF was up-regulated and maintained at high level after 48h, immunopositive cells were mainly neutrophils, neurons and endothelial cells [Figure - 10].


 ╗ Discussion Top


Animal model

Several experimental models of ICH have been described.[8],[9],[10] With modification of previous ones, here we developed an ICH rat model by sampling the blood from the tail artery instead of the femoral artery. The modifications made the model more reliable since the procedure had no effect on the neurobehavioral evaluation.

Brain water content and neurobehavioral evaluation

We demonstrated that the brain edema reached its peak between 48h and 72h and resolved at seven days after ICH, consistent with that of the neurobehavioral deficit scores. This suggested that there was a possible correlation between brain edema and motor behavior changes. Since four neurobehavioral tests were used for evaluation of the motor behavior, the neurobehavioral deficit scores should be more reliable.

Inflammatory cytokines: TNF-a, IL-6, ICAM-1 and NF-kB

Neutrophilic inflammation is in the vicinity of cerebral hematoma. Neutrophils release a variety of cytokines which might play an important role in brain edema formation and aggravation, such as TNF-a, ICAM-1, IL-6 and NF-kB.[11],[12],[13] It is also characterized by leukocyte behavior changes in the microvessels. Many leukocytes roll and adhere to the postcapillary venule and capillary walls and then these neutrophils infiltrate and migrate outside the vascular walls and move into the parenchyma. This inflammatory response is associated with expression of inflammatory mediators, including inflammatory cytokines,[14] chemokines[15] and adhesion molecules.[16] In turn, infiltrated neutrophils release proteases and oxidases and result in secondary brain injury.[17] Our results indicated that neutrophils around the hematoma were the most abundant at 48h to 72h postoperation; the amount of inflammatory cytokines such as IL-6 and TNF-a increased and reached their peaks at 48h after ICH. The expression of ICAM-1 was up-regulated, correlated with the action of IL-6 and TNF-a after ICH. Additionally the immunopositive ICAM-1 neurons were the most abundant at 72h. ICAM-1 neurons might contribute to the adhesion of activated microglias to neurons, leading to the neuron injury and brain edema after ICH. Transcription factor NF-kB plays a key role in secondary impairments of the tissues around the hematoma[18],[19] by inducing the expression of various genes related to cell injury and apoptosis. Here we demonstrated that the expression of NF-kB was consistent with the time course of brain edema after ICH, suggesting that NF-kB may worsen the brain edema and brain injury.

Complement system: C3 and CR2

C3 is an indicator of complement activation,[20] C3d is a degradation product of activated C3. CR2 is a receptor for C3d. It belongs to a family of complement regulatory proteins and is known for its bridging function at the intersection of innate host defense and acquired humoral immunity.[21] Possibly by the adjuvant effect of C3d, the C system (C3 and CR2) may be involved in selecting antigens for recognition by the acquired immune system. This selecting antigen function is an immunity-augmenting function. The enhancement magnitude of antigen attachment of C3d might be 10,000-fold,[21],[22] which is far greater than that of complete Freund's adjuvant. It showed that local activation of complement in the brain is of pathophysiological significance in both degenerative and inflammatory neurological diseases including cerebrovascular disorders. Complement depletion significantly reduced edema formation at both 24 and 72h after ICH.[23],[24] We demonstrated the up-regulation of C3 and CR2 protein levels in response to ICH in rat brain, suggesting C3 and CR2 may play a role in the development of inflammation, brain edema and brain injury after ICH.

VEGF

The results showed that the VEGF in rat brain after ICH was up-regulated. The up-regulation of VEGF correlates with blood-brain barrier breakdown and vasogenic brain edema formation. VEGF expression reached its peak at 72h. However, many positive cells, small vessel and vessel-like structures were still observed at seven days, suggesting that VEGF promoted the late stage of vasogenic edema. VEGF accelerates the breakdown of endothelial cells and the basilar membrane, which could subsequently increase the leukocyte infiltration and aggravate brain edema,[25] VEGF might stimulate the angiogenic response and neovascularization.[26]


 ╗ Conclusion Top


This study characterized the brain edema, neurobehavioral deficit and inflammatory response after ICH in rat. Our results showed that cytokines, complement system and blood-brain barrier breakdown might play a role in the development of the brain edema and neuron injury after ICH. Our work will help to shed light on understanding of how the brain modulates inflammatory injury responses.


 ╗ Acknowledgment Top


We would like to thank technician Hongran Wu and Bianfen Jin for their technical assistance.

 
 ╗ References Top

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    Figures

[Figure - 1], [Figure - 2], [Figure - 3], [Figure - 4], [Figure - 5], [Figure - 6], [Figure - 7], [Figure - 8], [Figure - 9], [Figure - 10]

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23 Bauhinia championiiExtraction Treatment of Collagen-Induced Arthritis via Downregulation of the Expression of TLR4, MyD88 and NF-?B
Wei Xu,Kedan Chu,Huang Li,Yuqin Zhang,Mingqing Huang,Haiyin Zheng,Mei Sha,Xun Zhang,Lidian Chen
The American Journal of Chinese Medicine. 2013; 41(02): 379
[Pubmed] | [DOI]
24 Effects of shuangtengbitong tincture on collagen-induced arthritis in rats
WEI XU,HUANG LI,KEDAN CHU,YUQIN ZHANG,HAIYIN ZHENG,MINGQING HUANG,XUN ZHANG,LIDIAN CHEN
Molecular Medicine Reports. 2013; 8(5): 1479
[Pubmed] | [DOI]
25 Alpha-7 nicotinic acetylcholine receptor agonists in intracerebral hemorrhage: An evaluation of the current evidence for a novel therapeutic agent
Sussman, E.S. and Kellner, C.P. and McDowell, M.M. and Bruce, S.S. and Heuts, S.G. and Zhuang, Z. and Bruce, R.A. and Claassen, J. and Connolly Jr., E.S.
Neurosurgical Focus. 2013; 34(5)
[Pubmed]
26 Bauhinia championii extraction treatment of collagen-induced arthritis via downregulation of the expression of TLR4, MyD88 and NF-╬║B
Xu, W. and Chu, K. and Li, H. and Zhang, Y. and Huang, M. and Zheng, H. and Sha, M. and Zhang, X. and Chen, L.
American Journal of Chinese Medicine. 2013; 41(2): 379-390
[Pubmed]
27 Effect of pretreatment with a tyrosine kinase inhibitor (PP1) on brain oedema and neurological function in an automated cortical cryoinjury model in mice
Turel, M.K. and Moorthy, R.K. and Sam, G.A. and Samuel, P. and Murthy, M. and Babu, K.S. and Rajshekhar, V.
Journal of Clinical Neuroscience. 2013; 20(4): 593-596
[Pubmed]
28 Association of molecular markers with perihematomal edema and clinical outcome in intracerebral hemorrhage
Li, N. and Liu, Y.F. and Ma, L. and Worthmann, H. and Wang, Y.L. and Wang, Y.J. and Gao, Y.P. and Raab, P. and Dengler, R. and Weissenborn, K. and Zhao, X.Q.
Stroke. 2013; 44(3): 658-663
[Pubmed]
29 ApolipoproteinE mimetic peptides improve outcome after focal ischemia
Wang, H. and Anderson, L.G. and Lascola, C.D. and James, M.L. and Venkatraman, T.N. and Bennett, E.R. and Acheson, S.K. and Vitek, M.P. and Laskowitz, D.T.
Experimental Neurology. 2013; 241(1): 67-74
[Pubmed]
30 Toll-like receptor 4 signaling in intracerebral hemorrhage-induced inflammation and injury
Fang, H. and Wang, P.-F. and Zhou, Y. and Wang, Y.-C. and Yang, Q.-W.
Journal of Neuroinflammation. 2013; 10(27)
[Pubmed]
31 Effect of ghrelin on brain edema induced by acute and chronic systemic hypoxia
Hossienzadeh, F. and Babri, S. and Alipour, M.R. and Ebrahimi, H. and Mohaddes, G.
Neuroscience Letters. 2013; 534(1): 47-51
[Pubmed]
32 Mechanism of focal cerebral ischemic tolerance in rats with ischemic preconditioning involves MyD88- and TRIF-dependent pathways
HAN LI,MINGYUE JIN,TAO LV,JUNHONG GUAN
Experimental and Therapeutic Medicine. 2013; 6(6): 1375
[Pubmed] | [DOI]
33 Inflammation in intracerebral hemorrhage: From mechanisms to clinical translation
Yu Zhou,Yanchun Wang,Jian Wang,R. Anne Stetler,Qing-Wu Yang
Progress in Neurobiology. 2013;
[Pubmed] | [DOI]
34 The Molecular Mechanisms that Promote Edema After Intracerebral Hemorrhage
Daniel Bodmer, Kerry A. Vaughan, Brad E. Zacharia, Zachary L. Hickman, E. Sander Connolly
Translational Stroke Research. 2012;
[VIEW] | [DOI]
35 Chemokines and Their Receptors in Intracerebral Hemorrhage
Yao Yao, Stella E. Tsirka
Translational Stroke Research. 2012;
[VIEW] | [DOI]
36 Lithium pretreatment reduces brain injury after intracerebral hemorrhage in rats
K Kang,Y-J Kim,Y-H Kim,J N Roh,J-M Nam,P-Y Kim,W-S Ryu,S-H Lee,B-W Yoon
Neurological Research. 2012; 34(5): 447
[Pubmed] | [DOI]
37 Perivascular and perineural extension of formed and soluble blood elements in an intracerebral hemorrhage rat model
GuoLin He, TianMing LŘ, BingXun Lu, Duan Xiao, Jia Yin, XiaoJia Liu, Guang Qiu, Min Fang, YuanYuan Wang
Brain Research. 2012;
[VIEW] | [DOI]
38 Effect of two Chinese medicinal compounds, blood-activating and water-draining medicine, on tumor necrosis factor α and nuclear factor κB expressions in rats with intracerebral hemorrhage
Yu-bo Li, Xiang-ning Cui, Yan Li, Lin Pan, Jian-yan Wen
Chinese Journal of Integrative Medicine. 2012;
[VIEW] | [DOI]
39 Age-Related Comparisons of Evolution of the Inflammatory Response After Intracerebral Hemorrhage in Rats
Starlee Lively, Lyanne C. Schlichter
Translational Stroke Research. 2012;
[VIEW] | [DOI]
40 Transplantation of adipose-derived stem cells is associated with neural differentiation and functional improvement in a rat model of intracerebral hemorrhage
Chen, J. and Tang, Y.-X. and Liu, Y.-M. and Chen, J. and Hu, X.-Q. and Liu, N. and Wang, S.-X. and Zhang, Y. and Zeng, W.-G. and Ni, H.-J. and Zhao, B. and Chen, Y.-F. and Tang, Z.-P.
CNS Neuroscience and Therapeutics. 2012; 18(10): 847-854
[Pubmed]
41 The protective role of oxymatrine on neuronal cell apoptosis in the hemorrhagic rat brain
Huang, M. and Hu, Y.-Y. and Dong, X.-Q. and Xu, Q.-P. and Yu, W.-H. and Zhang, Z.-Y.
Journal of Ethnopharmacology. 2012; 143(1): 228-235
[Pubmed]
42 Changes in content of glycolipids, sphingosine and cytokines in the rat brain with experimental edema
Zakaryan, G.V.
UkrainŠskyi Biokhimichnyi Zhurnal. 2012; 84(4): 70-73
[Pubmed]
43 Lithium pretreatment reduces brain injury after intracerebral hemorrhage in rats
Kang, K. and Kim, Y.-J. and Kim, Y.-H. and Roh, J.N. and Nam, J.-M. and Kim, P.-Y. and Ryu, W.-S. and Lee, S.-H. and Yoon, B.-W.
Neurological Research. 2012; 34(5): 447-454
[Pubmed]
44 Transplantation of Adipose-Derived Stem Cells is Associated with Neural Differentiation and Functional Improvement in a Rat Model of Intracerebral Hemorrhage
Juan Chen,Ying-Xin Tang,Yong-Ming Liu,Ji Chen,Xiao-Qing Hu,Na Liu,Shu-Xin Wang,Yu Zhang,Wen-Gao Zeng,Hou-Jie Ni,Bin Zhao,Yan-Fang Chen,Zhou-Ping Tang
CNS Neuroscience & Therapeutics. 2012; 18(10): 847
[Pubmed] | [DOI]
45 The protective role of oxymatrine on neuronal cell apoptosis in the hemorrhagic rat brain
Man Huang,Yue-Yu Hu,Xiao-Qiao Dong,Qiu-Ping Xu,Wen-Hua Yu,Zu-Yong Zhang
Journal of Ethnopharmacology. 2012; 143(1): 228
[Pubmed] | [DOI]
46 Dexamethasone inhibits ICAM-1 and MMP-9 expression and reduces brain edema in intracerebral hemorrhagic rats
Jen-Tsung Yang, Tsong-Hai Lee, I-Neng Lee, Chiu-Yen Chung, Chia-Hui Kuo, Hsu-Huei Weng
Acta Neurochirurgica. 2011;
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47 Comparison of humoral neuroinflammation and adhesion molecule expression in two models of experimental intracerebral hemorrhage
Arthur Liesz, Moritz Middelhoff, Wei Zhou, Simone Karcher, Sergio Illanes, Roland Veltkamp
Experimental & Translational Stroke Medicine. 2011; 3(1): 11
[VIEW] | [DOI]
48 Study of cytokines content and gangliosides metabolism at experimental brain edema
Zakaryan, A.V. and Kazaryan, G.S. and Zakaryan, G.V. and Melkonyan, M.M. and Hovsepyan, L.M.
Biomeditsinskaya Khimiya. 2011; 57(4): 455-460
[Pubmed]
49 Complement Factor H Y402H polymorphism is associated with an increased risk of mortality after intracerebral hemorrhage
Geoffrey Appelboom, Matthew Piazza, Brian Y. Hwang, Samuel Bruce, Steve Smith, Alexander Bratt, Emilia Bagiella, Neeraj Badjatia, Stephan Mayer, E. Sander Connolly
Journal of Clinical Neuroscience. 2011;
[VIEW] | [DOI]
50 Ginkgolide B reduces neuronal cell apoptosis in the hemorrhagic rat brain: Possible involvement of Toll-like receptor 4/nuclear factor-kappa B pathway
Yue-Yu Hu, Man Huang, Xiao-Qiao Dong, Qiu-Ping Xu, Wen-Hua Yu, Zu-Yong Zhang
Journal of Ethnopharmacology. 2011;
[VIEW] | [DOI]
51 The study of cytokine content and ganglioside metabolism in experimental brain edema
A. V. Zakaryan, G. S. Kazaryan, G. V. Zakaryan, M. M. Melkonyan, L. M. Hovsepyan
Biochemistry (Moscow) Supplement Series B Biomedical Chemistry. 2011; 5(1): 51-54
[Pubmed] | [DOI]
52 Myocardial Infarction and Intracerebral Hemorrhage in a Chinese Population: Relationship with Lipoproteins and Adipokines
Jessica Smith,Zhenjun Liu,Huiling Lu,Daowen Wang,Katherine Cianflone
Chinese Medicine. 2010; 01(03): 69
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53 Heat shock protein 70 upregulation by geldanamycin reduces brain injury in a mouse model of intracerebral hemorrhage
Manaenko, A. and Fathali, N. and Chen, H. and Suzuki, H. and Williams, S. and Zhang, J.H. and Tang, J.
Neurochemistry International. 2010; 57(7): 844-850
[Pubmed]
54 Role of oxidative stress and inflammation in hypoxia-induced cerebral edema: A molecular approach
Himadri, P. and Kumari, S.S. and Chitharanjan, M. and Dhananjay, S.
High Altitude Medicine and Biology. 2010; 11(3): 231-244
[Pubmed]
55 Heat shock protein 70 upregulation by geldanamycin reduces brain injury in a mouse model of intracerebral hemorrhage
Manaenko, A., Fathali, N., Chen, H., Suzuki, H., Williams, S., Zhang, J.H., Tang, J.
Neurochemistry International. 2010; 57(7): 844-850
[Pubmed]
56 Heat shock protein 70 upregulation by geldanamycin reduces brain injury in a mouse model of intracerebral hemorrhage
Anatol Manaenko,Nancy Fathali,Hank Chen,Hidenori Suzuki,Shammah Williams,John H. Zhang,Jiping Tang
Neurochemistry International. 2010; 57(7): 844
[Pubmed] | [DOI]
57 Role of Oxidative Stress and Inflammation in Hypoxia-Induced Cerebral Edema: A Molecular Approach
P. Himadri,Sarada S. Kumari,M. Chitharanjan,S. Dhananjay
High Altitude Medicine & Biology. 2010; 11(3): 231
[Pubmed] | [DOI]
58 Time course of plasma leptin concentrations after acute spontaneous basal ganglia hemorrhage
Dong, X.-Q., Huang, M., Hu, Y.-Y., Yu, W.-H., Zhang, Z.-Y.
World Neurosurgery. 2010; 74((2-3)): 286-293
[Pubmed]
59 Time Course of Plasma Leptin Concentrations After Acute Spontaneous Basal Ganglia Hemorrhage
Xiao-Qiao Dong,Man Huang,Yue-Yu Hu,Wen-Hua Yu,Zu-Yong Zhang
World Neurosurgery. 2010; 74(2-3): 286
[Pubmed] | [DOI]
60 Role of oxidative stress and inflammation in hypoxia-induced cerebral edema: A molecular approach
Himadri, P., Kumari, S.S., Chitharanjan, M., Dhananjay, S.
High Altitude Medicine and Biology. 2010; 11(3): 231-244
[Pubmed]
61 Correlation of vascular endothelial growth factor to permeability of blood-brain barrier and brain edema during high-altitude exposure
Zhou, Q., Liu, C., Wang, J., Wang, Y., Zhou, B.
Neural Regeneration Research. 2009; 4(10): 775-779
[Pubmed]
62 High concentrations of procoagulant microparticles in the cerebrospinal fluid and peripheral blood of patients with acute basal ganglia hemorrhage are associated with poor outcome
Huang, M., Hu, Y.-Y., Dong, X.-Q.
Surgical Neurology. 2009; 72(5): 481-489
[Pubmed]
63 The complement cascade as a therapeutic target in intracerebral hemorrhage
Ducruet, A.F., Zacharia, B.E., Hickman, Z.L., Grobelny, B.T., Yeh, M.L., Sosunov, S.A., Connolly Jr., E.S.
Experimental Neurology. 2009; 219(2): 398-403
[Pubmed]
64 Correlation of macrophage inflammatory protein-2 expression and brain edema in rats after intracerebral hemorrhage
Wu, H., Cong, Y., Wang, D., Zhao, R., Qi, J.
International Journal of Clinical and Experimental Pathology. 2009; 2(1): 83-90
[Pubmed]
65 Brain edema after intracerebral hemorrhage in rats: The role of iron overload and aquaporin 4 - Laboratory investigation
Wang, G.Q., Yang, Q.D., Tang, Q.P., Li, G.L., Li, D.F., Hu, W.M., Xia, L., Pei, Y.H.
Journal of Neurosurgery. 2009; 110(3): 462-468
[Pubmed]
66 Pharmacogenomic effects of apolipoprotein e on intracerebral hemorrhage
James, M.L., Sullivan, P.M., Lascola, C.D., Vitek, M.P., Laskowitz, D.T.
Stroke. 2009; 40(2): 632-639
[Pubmed]
67 High concentrations of procoagulant microparticles in the cerebrospinal fluid and peripheral blood of patients with acute basal ganglia hemorrhage are associated with poor outcome
Man Huang,Yue-Yu Hu,Xiao-Qiao Dong
Surgical Neurology. 2009; 72(5): 481
[Pubmed] | [DOI]
68 Oxymatrine Downregulates TLR4, TLR2, MyD88, and NF- B and protects rat brains against focal ischemia
Zhang, X., Fan, H., Li, L., Liu, Y., Yang, C., Yang, Y., Yin, J.
Mediators of Inflammation. 2009; 704706
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69 The complement cascade as a therapeutic target in intracerebral hemorrhage
Andrew F. Ducruet,Brad E. Zacharia,Zachary L. Hickman,Bartosz T. Grobelny,Mason L. Yeh,Sergey A. Sosunov,E. Sander Connolly
Experimental Neurology. 2009; 219(2): 398
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70 Brain edema after intracerebral hemorrhage in rats: the role of iron overload and aquaporin 4
Wang Gai Qing,Yang Qi Dong,Tang Qing Ping,Li Guang Lai,Li Dong Fang,Hu Wei Min,Lian Xia,Pei Yu Heng
Journal of Neurosurgery. 2009; 110(3): 462
[Pubmed] | [DOI]
71 Oxymatrine Downregulates TLR4, TLR2, MyD88, and NF-?B and Protects Rat Brains against Focal Ischemia
Hongguang Fan,Litao Li,Xiangjian Zhang,Ying Liu,Chenhui Yang,Yi Yang,Jing Yin
Mediators of Inflammation. 2009; 2009: 1
[Pubmed] | [DOI]
72 Changes of Blood Flow Perfusion by MR and NF-?▀ Expression in the Region of Perihematoma after Experimental Intracerebral Hemorrhage: A Correlation Study
Dan He,Lin Zhao,Linfang Li,Huaijun Liu,Lihong Zhang,David T. Yew
International Journal of Neuroscience. 2009; 119(6): 806
[Pubmed] | [DOI]
73 Changes of blood flow perfusion by MR and NF-╬║╬▓ expression in the region of perihematoma after experimental intracerebral hemorrhage: A correlation study
He, D. and Zhao, L. and Li, L. and Liu, H. and Zhang, L. and Yew, D.T.
International Journal of Neuroscience. 2009; 119(6): 806-814
[Pubmed]
74 Rosiglitazone, a PPAR gamma agonist, attenuates inflammation after surgical brain injury in rodents
Amy Hyong,Vikram Jadhav,Steve Lee,Wenni Tong,Jamaine Rowe,John H. Zhang,Jiping Tang
Brain Research. 2008; 1215: 218
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75 Rosiglitazone, a PPAR gamma agonist, attenuates inflammation after surgical brain injury in rodents
Hyong, A., Jadhav, V., Lee, S., Tong, W., Rowe, J., Zhang, J.H., Tang, J.
Brain Research. 2008; 1215 C: 218-224
[Pubmed]
76 Cerebral angiogenesis after collagenase-induced intracerebral hemorrhage in rats
Tang, T., Liu, X.-J., Zhang, Z.-Q., Zhou, H.-J., Luo, J.-K., Huang, J.-F., Yang, Q.-D., Li, X.-Q.
Brain Research. 2007; 1175(1): 134-142
[Pubmed]
77 Cerebral angiogenesis after collagenase-induced intracerebral hemorrhage in rats
Tao Tang,Xiao-Juan Liu,Zong-Qi Zhang,Hua-Jun Zhou,Jie-Kun Luo,Ju-Fang Huang,Qi-Dong Yang,Xing-Qun Li
Brain Research. 2007; 1175: 134
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78 Inflammation in intracerebral hemorrhage: Clearly present, but what is its role?
Butcher, K.
Neurology India. 2006; 54(4): 352-353
[Pubmed]



 

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