| ORIGINAL ARTICLE
|Year : 2013 | Volume
| Issue : 6 | Page : 606--609
Matrix metalloproteinases and their tissue inhibitors in serum and cerebrospinal fluid of patients with moderate and severe traumatic brain injury
Kebin Zheng1, Chunhui Li2, Xiaosong Shan2, Haipeng Liu2, Wufang Fan1, Zhenshan Wang1, Ping Zheng3
1 Department of Neuroscience, College of Life Science, Hebei University, Hebei Province, China
2 Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
3 Department of Neurosurgery, Shanghai Pudong New area People's Hospital, 201299, Shanghai, China
Objective: In this study, we investigated matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMPs) in cerebrospinal fluid (CSF) and plasma of traumatic brain injury (TBI) patients. Patients and Methods: A total of 30 patients with moderate and severe TBI and 15 age-matched controls were enrolled in this study. Plasma and CSF samples were collected within 24 h (as the initial value), at 72 and 120 h post injury. CSF and plasma MMP-9, MMP-2, TIMP-1 and TIMP-2 were estimated using ELISA. Different levels of these indexes were compared in the two groups and further investigated the correlation between each other. Results: There was a significant elevation in the levels of the initial MMP-9 in the CSF (P < 0.05), which lasted for 72 h post injury. TIMP-1 kept increasing within 120 h post injury and it was different compared with TIMP-1 at 24 and 72 h post injury. Plasma levels of MMP-9, MMP-2, TIMP-1 and TIMP-2 in TBI patients were also significantly different from those in controls. Furthermore the CSF MMP-9 in patients with severe TBI was higher than that in patients with moderate TBI. In addition, there was a positive relationship between the initial MMP-9 and TIMP-1 at 120 h post injury (r = 0.614, P < 0.01). Conclusion: MMPs and TIMPs are increased in both CSF and plasma of TBI patients. TIMP-1 has a positive correlation with MMP-9 and the initial MMP-9 is associated with the neurological outcomes.
College of Life Science, Hebei University
Source of Support: Supported by the Natural Science Foundation of Hebei Province (No. 2012201136) and Medical Science Special Foundation of Hebei University (No. 2012B2004), Conflict of Interest: Hebei postdoctoral fund (No. 111867).
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