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 ORIGINAL ARTICLE
Year : 2004  |  Volume : 52  |  Issue : 3  |  Page : 359--362

Demonstration of IgG antibodies to 30 Kd protein antigen in CSF for diagnosis of tuberculous meningitis by antibody-capturing ELISA


1 Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur - 440 010, India
2 Environmental Modeling and Genomic division, Nagpur - 440 010, India

Correspondence Address:
H F Daginawala
Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur - 440 010
India
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Source of Support: None, Conflict of Interest: None


PMID: 15472427

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Objective: A simple and rapid immunological assay method has been developed to demonstrate the presence of IgG antibodies to 30Kd protein antigen (30Kdpa) and culture filtrate protein (CFP) in the CSF of patients with Tuberculous meningitis (TBM). Method: Antibody capturing Enzyme Linked Immunosorbent Assay (ELISA) was standardized with CFP antigen of MTB. The IgG antibodies were assayed in CSF sample from TBM and non-TBM patients against 30 Kdpa. Results: The sensitivity and specificity of IgG antibodies for the diagnosis of suspected patients of TBM using 30 Kdpa was 80% and 91% respectively and the corresponding figures for CFP were 85% and 94% respectively. The sensitivity and specificity in two confirmed cases of TBM was 100%. Conclusion: The presence of this 30Kdpa in the CSF of suspected cases of TBM consistently would indicate that the selected protein band carries the candidate protein marker antigen, which is specific to M. tuberculosis and could be considered as a diagnostic marker for TBM.






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Online since 20th March '04
Published by Wolters Kluwer - Medknow