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| ORIGINAL ARTICLE |
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| Year : 2011 | Volume
: 59
| Issue : 4 | Page : 513--520 |
Immunohistochemical differentiation of inflammatory myopathies
Jayalakshmi B Panicker1, Geeta Chacko1, Anil Kumar B Patil2, Mathew Alexander2, Jayaprakash Muliyil3
1 Section of Neuropathology, Department of Neurological Sciences and Pathology, Christian Medical College, Vellore, India
2 Section of Neurology, Department of Neurological Sciences, Christian Medical College, Vellore, India
3 Section of Neurology, Department of Community Health, Christian Medical College, Vellore, India
Correspondence Address:
Geeta Chacko
Section of Neuropathology, Department of Neurological Sciences and Pathology, Christian Medical College, Vellore - 632 004
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0028-3886.84329
Background: Idiopathic inflammatory myopathies are a heterogeneous group of acquired muscle disorders with considerable overlap in the histological features, making histological diagnosis difficult at times. Aims: To determine the immunohistochemical profile of clinically suspected cases of inflammatory myopathies, using monoclonal antibodies to HLA-1 and membrane attack complex (MAC), and to correlate the clinical, serological, and electromyographic profile and the histopathological picture, with the immunohistochemical profile. Settings and Design: This was a retrospective study analyzing the clinical and histopathological features in muscle of clinically suspected cases of inflammatory myopathy and correlating it to their HLA-1 and MAC immunostaining profiles. Material and Methods: The study subjects included 33 cases with suspected inflammatory myopathy and 59 with non-inflammatory muscle disease, as controls. Clinical data, electromyographic findings, serological profile, and details of therapy were obtained from patient records. Statistical Analysis: Student 'T' test, Pearson's Chi square test, and Kappa statistics were used appropriately. Results: Although HLA-1 and MAC immunostaining did not help to differentiate the individual subtypes of inflammatory myopathy, when either HLA-1 or MAC was positive, inflammatory myopathy could be ruled in with 86.5% certainty and when both HLA-1 and MAC were negative, it could be ruled out with 95% certainty. Conclusions: A combination of clinical presentation, serological profile, electromyographic and histopathological features, together with the immunoprofile for HLA-1 and MAC, contribute toward making a diagnosis of inflammatory myopathy.
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