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 ORIGINAL ARTICLE
Year : 2014  |  Volume : 62  |  Issue : 1  |  Page : 53--56

Relative quantitative expression of hypoxia-inducible factor 1 alpha messenger ribonucleic acid in recurrent craniopharyngiomas


Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China

Correspondence Address:
Jianguo Xu
Department of Neurosurgery, West China Hospital, Sichuan University, 37 Guoxue Street, Chengdu, Sichuan, 610041
China
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Source of Support: This work was supported by Grant No. 3097382 and No. 81172411 from National Natural Science Foundation of China and China Medical Board of New York, Conflict of Interest: None


DOI: 10.4103/0028-3886.128291

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Background: Craniopharyngioma is a benign tumor, but recurrences are common and prognosis is poor. The pathologic mechanism underlying the high recurrence is still unknown. Aims: We hypothesized that there are hypoxic microenvironments within craniopharyngiomas and hypoxia inducible factor-1 alpha (HIF-1α) and its related genes are largely expressed in recurrent craniopharyngiomas. Materials and Methods: A total of 19 patients with craniopharyngiomas have been identified. The relative quantitative expressions of HIF-1α, vascular endothelial growth factor (VEGF) and carbonic adhydrase 9 (CA9) messenger ribonucleic acid (mRNA) of craniopharyngioma tissues were detected by real time reverse transcription polymerase chain reaction. Results: HIF-1α and VEGF mRNA was significantly up-regulated in recurrent craniopharyngiomas. Mean expression levels (recurrent craniopharyngiomas compared with non-recurrent craniopharyngiomas, as normalized to expression of β-actin) were 3.09 versus 0.75 (P = 0.001) for HIF-1α, 1.07 versus 0.32 (P = 0.000) for VEGF, 1.21 versus 1.93 (P = 0.503) for CA9. In craniopharyngiomas, the expression of VEGF showed a significant correlation with HIF-1α (r = 0.836, P = 0.000). Conclusion: There were hypoxic microenvironments within craniopharyngiomas. Therefore, preventing the tumor cells from adapting to the hypoxic conditions may be an effective way to obviate the relapse of craniopharyngioma.






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