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|Year : 2021 | Volume
| Issue : 4 | Page : 856-860
Glioblastoma with Primitive Neuroectodermal Component Treated with Adjuvant Radiotherapy and Temozolomide: A Pooled Analysis of 23 Patients
Rony Benson1, Supriya Mallick1, Suvendu Purkait2, KP Haresh1, Subhash Gupta1, Mehar Chand Sharma2, Vaishali Suri2, Dayanand Sharma1, GK Rath1
1 Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi, India
2 Department of Pathology, All India Institute of Medical Sciences, New Delhi, India
|Date of Submission||28-Apr-2016|
|Date of Decision||15-Jun-2016|
|Date of Acceptance||05-Jan-2017|
|Date of Web Publication||14-Aug-2021|
Dr. Subhash Gupta
Department of Radiation Oncology, All India Institute of Medical Sciences, New Delhi
Source of Support: None, Conflict of Interest: None
Aim: Glioblastoma (GBM) is one of the most aggressive neoplasms of the central nervous system with dismal survival. In recent years, different variants of GBM have been described in the literature. GBM with areas of neuroectodermal differentiation (GBM-PNET) is a relatively new entity in GBM. Presence of the neuroectodermal component increases the propensity of systemic dissemination as with other intracranial primitive neuroectodermal tumors (PNET). The optimal treatment for these patients remains a controversy, with authors reporting local radiotherapy to craniospinal irradiation and chemotherapy. We intend to analyze the pattern of care for GBM with neuroectodermal component.
Materials and Methods: We retrieved data of four patients with GBM-PNET treated in our institute; data were also retrieved from published series to derive treatment and outcome results.
Results: In this series, we report the outcome of a series of four patients of GBM-PNET treated with adjuvant radiotherapy and temozolomide. All but one patient underwent gross total resection of the tumor. Adjuvant hypofractionated radiation with concurrent and adjuvant temozolomide was used in all cases. The median follow-up was 12.9 months in the present series. One patient experienced local recurrence 18 months after the treatment. A review of published literature on GBM-PNET was done; studies with details of patient outcome were used for an independent analysis. Twenty-three patients were identified, and the pooled analysis revealed a median progression free and overall survival of 10 and 25, months respectively. Extent of surgery, local radiation vs. craniospinal irradiation, and age at presentation had no impact on the survival.
Conclusion: GBM PNET is a new entity with only few cases reported so far. Clinical behavior and treatment outcome of these tumors are not different from conventional GBM. However, these patients are at higher risk of CSF dissemination. Hence, an individualized treatment approach is best suited.
Keywords: GBM-PNET, hypofractionated, radiotherapy, temozolomideKey Message: GBM PNET is a rare entity with higher rate of CSF dissemination. However, in CSF negative cases local RT may be sufficient along with Temozolomide.
|How to cite this article:|
Benson R, Mallick S, Purkait S, Haresh K P, Gupta S, Sharma MC, Suri V, Sharma D, Rath G K. Glioblastoma with Primitive Neuroectodermal Component Treated with Adjuvant Radiotherapy and Temozolomide: A Pooled Analysis of 23 Patients. Neurol India 2021;69:856-60
|How to cite this URL:|
Benson R, Mallick S, Purkait S, Haresh K P, Gupta S, Sharma MC, Suri V, Sharma D, Rath G K. Glioblastoma with Primitive Neuroectodermal Component Treated with Adjuvant Radiotherapy and Temozolomide: A Pooled Analysis of 23 Patients. Neurol India [serial online] 2021 [cited 2021 Oct 18];69:856-60. Available from: https://www.neurologyindia.com/text.asp?2021/69/4/856/323892
Glioblastoma (GBM) is the most common primary brain tumor occurring in patients more than 50 years of age. GBM with areas of neuroectodermal differentiation (GBM-PNET) is a rare entity with only few reported cases thus far. Maximal safe surgery is contemplated in all patients. However, adjuvant treatment in such patients varies widely from local radiotherapy to craniospinal irradiation and from concurrent and adjuvant temozolomide alone to platinum-based multiagent chemotherapy. Treatment outcomes of this new entity of GBM also varies across reports. Interestingly, some authors have reported better survival for these patients compared to GBM. Here, we intend to report the outcome of four patients of GBM-PNET treated in a tertiary care centre with hypofractionated simultaneous integrated boost intensity modulated radiotherapy (SIB-IMRT) with concurrent and adjuvant temozolomide. We also performed a systematic review of all published literature on GBM-PNET reporting treatment outcome in these patients, and analyzed the pooled data separately concerning survival and prognostic factors.
| » Materials and Methods|| |
We searched our institutional database from January 2008 to December 2015 and retrieved five cases. Central pathological review of these patients confirmed a diagnosis consistent with GBM-PNET in 4 patients; 1 patient was conformed to have GBM only. Demographic features and clinical characteristics, including radiological findings, surgical details, and histopathological features, were recorded in a proforma. In a quest to examine the behavior of these tumors, we retrieved all published reports pertaining to GBM-PNET. We retrieved six full-text articles and one abstract pertaining to the topic with 75 cases. However, individual patients and treatment data was not available for the largest series reported by Perry et al. and Lee et al. Hence, we pooled the survival data of our series of patients with the existing survival outcomes of 19 patients in the literature for a secondary analysis. Survival estimates were calculated using Kaplan–Meier method, and log rank test was used for univariate analysis. The Statistical Package for the Social Sciences version 16.0 (SPSS Inc., Chicago, IL, USA) was used for all statistical analysis.
Patient evaluation and treatment
Maximal safe surgery was attempted in all patients after neurological imaging was done. Once the histopathological diagnosis was confirmed as GBM-PNET, patients were subjected to contrast-enhanced magnetic resonance imaging (MRI) of the brain to look for residual disease, and cerebrospinal fluid (CSF) cytology and MRI spine to look for drop metastasis. Adjuvant radiation was delivered within 4–6 weeks of surgery. Radiation therapy was planned by intensity modulated radiotherapy with simultaneous boost (SIB-IMRT) technique. A thermoplastic immobilization cast was used for each patient for treatment position reproducibility. A contrast-enhanced computed tomography (CECT) scan was done in the treatment position for treatment planning. The low risk clinical target volume (CTV 50) included MRI abnormality visible on the T2 fluid-attenuated inversion recovery (FLAIR) sequences of the preoperative MRI with isotropic 1.5-cm margin. The high risk CTV (CTV 60) included a 1.5-cm margin around the T1 contrast enhancing tumor. An isotropic expansion of 5 mm was given around the clinical target volume to generate the planning target volume (PTV). A dose of 60 Gy in 20 fractions and 50 Gy in 20 fractions was prescribed for PTV60 and PTV50, respectively, using simultaneous integrated boost technique. Radiation was planned on the pinnacle treatment planning system. Concurrent temozolomide was prescribed at a dose of 75 mg/m2 daily in empty stomach following antiemetic and proton pump inhibitor (60–90 min before radiation). Temozolomide was continued for 2 weeks after completion of radiotherapy (radiotherapy was completed in 4 weeks). Adjuvant temozolomide was started after a gap of one month. First cycle was given at 150 mg/m2, and depending on the tolerance, was increased to 200 mg/m2 in the next cycle for six cycles repeated every four weeks.
Complete blood count and liver function tests were repeated once a week during radiation and before each cycle of adjuvant chemotherapy. Toxicity was charted according to Radiation Therapy Oncology Group (RTOG) acute toxicity grading criteria. All patients were reviewed after 4–6 weeks of completion of treatment with detailed clinical and neurological examination. Contrast-enhanced brain MRI was performed at 3 months of completion of radiotherapy, and subsequently, after every 3 months.
| » Results|| |
The patients were treated as a part of an ongoing prospective study comparing hypofractionated SIB-IMRT to conventionally fractionated, 3-dimensional conformal radiotherapy (3D-CRT) in patients with newly diagnosed GBM. Median age at diagnosis was 34 years (range: 23–42). The most common symptom was headache and vomiting seen in three patients followed by seizure in 2 patients. Median duration of symptoms before diagnosis was 9 months (range: 2–15). Two patients had frontal location of tumor, 1 had frontoparietal location, and one patient had temporal lobe lesion. The median Karnofsky performance status (KPS) was 90.
Contrast-enhanced MRI was done in all patients before surgery. The tumor was hypointense on T1 and hyperintense on T2. Three patients had tumor which was contrast enhancing, whereas in 1 patient the tumor was nonenhancing. The median tumor size was 5.2 cm (range: 3.8–6.8 cm). The preoperative diagnosis was high-grade glioma in 3 patients and low-grade glioma in one patient [Figure 1]. All patients underwent surgery in our institute with three patients undergoing gross total excision and one patient undergoing subtotal resection. Histopathological examination revealed a biphasic tumor, which composed of an astrocytic component and an undifferentiated small round cell component. The astrocytic component showed moderate increase in cellularity, marked pleomorphism with many tumor giant cells, increased mitosis, and necrosis suggestive of GBM with PNET-like areas [Figure 2]. Median MIB labeling index was 30 (range: 30–95). Immunohistochemistry of the glial component revealed p53 positivity in three cases. Isocitrate dehydrogenase (IDH-1) was positive in one patient and negative in three patients. The neuronal component showed synaptophysin positivity in all patients. Metastatic work up with CSF cytology and spine screening MRI showed no evidence of drop metastasis in any patient.
|Figure 1: Contrast-enhanced magnetic resonance imaging of a patient of GBM with areas of neuroectodermal differentiation (a) T1 axial noncontrast image (b-e) axial, coronal, and sagittal T1 postcontrast image showing heterogeneously contrast-enhancing mass with cystic component with mass effect and midline shift; (f and g) axial T2 FLAIR image; (h) axial diffusion-weighted image|
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|Figure 2: Photomicrographs showing a biphasic tumour comprising of astrocytic and primitive neuroectodermal components. The astrocytic areas showing oval to spindle cells, gemistocytes, and areas of necrosis with psedopallisading of cells (a and b, H and E ×400); Primitive neuroectodremal component is composed of small round to oval cells with hyperchromatic nuclei anf increase mitises (c, H and E ×400). Glial component is immunopositive for GFAP (d) but primitive neuroectodremal component is negative (e) and it is focally immunipositive for Neu N (f, ×400 each)|
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Adjuvant radiotherapy with concurrent temozolomide (75 mg/m2) was given to all the patients. Conformal radiotherapy at a dose of 60 Gy with intensity modulated radiotherapy by simultaneous integrated boost technique (50 Gy to the low risk PTV and 60 Gy to the high risk PTV) was given to all patients [Figure 3]. The patient and treatment characteristics are summarized in [Table 1]. The median follow-up was 12.9 months. One of the four patients developed local recurrence at 18 months while on follow up. All patients experienced grade III alopecia following radiotherapy and 1 patient developed grade 2 thrombocytopenia during adjuvant temozolomide.
|Figure 3: A 9-field dynamic intensity modulated radiotherapy plan for a patient of GBM with neuroectodermal differentiation|
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|Table 1: Summary of patient characteristics and immunohistochemistry findings and treatment outcome of patients treated with simultaneous integrated boost intensity modulated radiotherapy (SIB-IMRT) along with temozolomide (TMZ)|
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Twenty-three patients could be included in the pooled analysis. The pooled analysis revealed the median age at diagnosis as 47 years (range: 23–82). Six patients underwent gross total excision whereas 10 patients underwent subtotal excision; others underwent a biopsy or decompression only. Six patients underwent craniospinal irradiation (CSI) whereas 16 patients received focal radiation. All but two patients also received adjuvant temozolomide. The median MIB labeling index was 80 (range: 30–100). Only three patients had documented IDH-1 mutation and all the three patients were alive at the last follow-up. Median follow-up of the cohort was 13 months (range: 2–66). The survival analysis of the cohort revealed a median progression free and overall survival of 10 and 25 months, respectively [Figure 4]. The 2-year progression free survival and overall survival rate 23.4 and 52.1%, respectively. Univariate analysis for the prognostic variables including extent of surgery, local radiation vs. CSI, and age at presentation revealed no significant impact on survival.
|Figure 4: Kaplan–Meier survival estimate progression free survival and overall survival in the pooled cohort|
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| » Discussion|| |
GBM is the most common primary brain tumor occurring in patients more than 50 years of age and accounts for approximately 75% of all primary brain tumors in patients more than 60 years of age. Maximal safe surgery followed by adjuvant chemoradiation is the treatment of choice in GBM and produces a median survival of approximately 12–15 months., Different variants of GBM have been described in the literature viz. gliosarcoma, GBM with oligodendroglial component, small cell GBM, etc. In recent years, a new entity of GBM with PNET-like areas has emerged. GBM-PNET is a very rare entity of GBM and only few cases have been reported in literature so far. Radiologically, it is very difficult to differentiate high-grade gliomas from GBM-PNET, which usually presents with a solid cystic lesion with heterogeneous contrast enhancement. In a small series, Ali et al. reported substantially reduced apparent diffusion coefficient (ADC) values in GBM-PNETs compared to conventional GBM. In the present series also, the preoperative diagnosis was high-grade glioma in 3 patients whereas 1 patient had a preoperative diagnosis of low-grade glioma. These tumors are well-circumscribed and may facilitate surgical excision. However, histopathological examination reveals a biphasic tumor composed of an astrocytic component and undifferentiated small round cell component suggestive of GBM-PNET. The astrocytic areas show nuclear atypia and necrosis, whereas the neuroectodermal foci show high mitotic activity with rosette formation. The glial component usually shows glial fibrillary acid protein (GFAP) positivity, whereas the neuroectodermal foci show positivity for neuronal markers such as synaptophysin and chromogranin. The glial component may show variable positivity to p53, IDH-1, and epithelial growth factor receptor (EGFR). In the present series, 3 out of 4 cases showed p53 mutation, and only 1 showed IDH-1 mutation corroborating the findings of a similar series published by Song et al.
Maximal safe resection is considered optimum for GBM and is advocated for this new entity as well. CSF and systemic spread is rare in GBM, however, it is common in PNETs, which brings into question the optimum adjuvant therapy for GBM-PNET. Systemic metastasis has rarely been reported from a GBM-PNET, however, there are reports of CSF spread in approximately 15–20% of the cases. Thus, evaluation to detect CSF spread such as contrast-enhanced MRI of the spine and CSF cytology should be done in all patients.
Adjuvant radiation volume has been an area lacking consensus among various centers, with volume ranging from craniospinal irradiation to local radiotherapy. There is consensus that patients with CSF positivity need to be treated with craniospinal irradiation. The toxicity associated with craniospinal irradiation may be of concern when considering CSI in patients with CSF negative disease. In the largest series by Perry et al., 17 patients received local radiotherapy whereas 1 patient received craniospinal irradiation. Another series from O'Leary et al. reported the feasibility of craniospinal irradiation along with concurrent temozolomide in these patients with survival not different from focally treated GBM.
Chemotherapy regimen also varies widely with some series using platinum-based chemotherapy, as in PNET, while others using temozolomide-based chemotherapy alone, as in GBM. One series by Lee et al. used a combination of temozolomide and platinum chemotherapy and found it to be a feasible option. In our series, we used local radiotherapy along with concurrent and maintenance temozolomide.
Median survival of these patients ranged from 9 to 10 months according to the published literature. Some authors have reported the outcome in patients with GBM-PNET to be better than that of the GBM patients. Our pooled analysis revealed a median progression free survival and overall survival of 10 and 25 months, respectively. The well-circumscribed nature of the disease may facilitate better surgical resection and confer better survival. In addition, these tumors may harbor more IDH-1 mutation or MGMT methylation. A summary of various case series, their adjuvant treatment approach, and survival outcomes is summarized in [Table 2].,,,,,,
|Table 2: Summary of adjuvant treatment and survival outcome patients of glioblastoma with neuroectodermal differentiation from various published case series|
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| » Conclusion|| |
Based on the current available evidence, it is difficult for us to conclude what regimen of chemotherapy and what volume of irradiation is ideal among these patients. The adjuvant treatment needs to be individualized considering the good outcome in patients treated with adjuvant local radiation and concurrent temozolomide, with or without platinum-based chemotherapy. The survival outcome in these patients may be slightly better in patients with GBM-PNET than in patients with GBM.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2]