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| ORIGINAL ARTICLE |
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| Year : 2022 | Volume
: 70
| Issue : 2 | Page : 530--534 |
The Effect of Green Tea with EGCG Active Compound in Enhancing the Expression of M2 Microglia Marker (CD206)
Abdulloh Machin1, Dinda Divamillenia2, Nurmawati Fatimah3, Imam Susilo4, D Agus Purwanto5, Imam Subadi6, Paulus Sugianto7, Muhammad Hamdan7, O Galuh Pratiwi2, Dyah Fauziah8, Kenia Izzawa9
1 Neurologist Consultant, Universitas Airlangga, Dr. Soetomo General Hospital, Universitas Airlangga Hospital, Surabaya, Inonesia
2 Undergraduate, Universitas Airlangga, Surabaya, Inonesia
3 Magister of Science, Universitas Airlangga, Surabaya, Indonesia
4 Anatomical Pathology Consultant, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia
5 Professor, Universitas Airlangga General Hospital, Surabaya, Indonesia
6 Physical Medicine and Rehabilitation Consultant, Dr. Soetomo General Hospital, Surabaya, Indonesia
7 Neurologist Consultant, Dr. Soetomo General Hospital, Surabaya, Indonesia
8 Anatomical Pathology Consultant, Universitas Airlangga, Surabaya, Indonesia
9 General Doctor, Universitas Airlangga, Dr. Soetomo General Hospital, Surabaya, Indonesia
Correspondence Address:
Dr. Dinda Divamillenia
Jojoran 1 Street Number 25, Mojo, Gubeng, Surabaya
Inonesia
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0028-3886.344631
Background: Stroke is a neurological deficit due to vascular disorders. Microglia are the first line of defense against brain injury. Anti-inflammatory cytokines activate M2 microglia, which upregulate CD206. EGCG is abundant in green tea, which has an anti-inflammatory effect.
Objective: To know the effect of green tea with its active compound EGCG on CD206 expression.
Settings and Design: True experimental trial design.
Material and Methods: Rattus Novergicus were divided into six groups: a negative control group (Sham), a positive control group (P0), MCAO mice given 10 mg/kg BW EGCG (P1), 20 mg/kg BW EGCG (P2), 30 mg/kg BW EGCG (P3), and 30 mg/kg BW standardized green tea extract (P4). CD206 expression was measured using immunohistochemistry and scored according to the Allred scoring guidelines.
Statistical Analysis Used: Descriptive test, Levine test, Kolmogorov–Smirnoff test, Independent sample t test, Pearson correlation test
Results: We discovered that there is a significant difference in CD206 expression between the Sham and P0 groups (P < 0.05). In addition, there are significant differences in expression between the sham group and the other two groups (P1 and P2) (P < 0.05). Furthermore, when we compared the P0 group with each treatment group, we found that CD206 expression between P0–P2, P0–P3, P0–P4 are significantly different. There is a significant correlation between green tea with its active compound EGCG and CD206 expression enhancement. The correlation is positive.
Conclusions: Green tea with EGCG active compound increases CD206 expression as an M2 marker in the Rattus norvegicus with MCAO model.
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