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TOPIC OF THE ISSUE: ORIGINAL ARTICLE
Year : 2010  |  Volume : 58  |  Issue : 5  |  Page : 727--731

Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis

Kusum Sharma1, Aman Sharma2, Malkit Singh1, Pallab Ray1, Ritika Dandora1, Shiv K Sharma1, Manish Modi3, Sudesh Prabhakar3, Meera Sharma1 
1 Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India
3 Department of Neurology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Kusum Sharma
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh
India

Background: Rapid and specific diagnosis of tubercular meningitis (TBM) is of utmost importance. Aim: To evaluate polymerase chain reaction (PCR) using protein b primers directed against M. tuberculosis for the diagnosis of tuberculous meningitis. Materials and Methods: PCR using protein b primers was performed in ten patients with confirmed TBM (culture positive), 60 patients with clinically suspected TBM and 40 patients with no TBM (control group). Results: Protein b PCR had a sensitivity of 90% and a specificity of 100% in patients with confirmed TBM. In 60 clinically diagnosed TBM patients, protein b PCR was positive in 49 (81.7%) patients. The overall sensitivity of microscopy, culture and PCR using protein b primers was 1.4%, 14.3%, and 82.8% and specificity was 100%, 100%, and 100% respectively. Conclusion: Protein b PCR is valuable in rapid diagnosis of TBM.


How to cite this article:
Sharma K, Sharma A, Singh M, Ray P, Dandora R, Sharma SK, Modi M, Prabhakar S, Sharma M. Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis.Neurol India 2010;58:727-731


How to cite this URL:
Sharma K, Sharma A, Singh M, Ray P, Dandora R, Sharma SK, Modi M, Prabhakar S, Sharma M. Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis. Neurol India [serial online] 2010 [cited 2022 Oct 1 ];58:727-731
Available from: https://www.neurologyindia.com/article.asp?issn=0028-3886;year=2010;volume=58;issue=5;spage=727;epage=731;aulast=Sharma;type=0