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Figure 3: Increased expression of HIF-1α is involved in IDH1-R132H-induced inhibition of glioblastoma malignancy.(a) Protein expression of HIF-1αin U87 and U251 cells transfected with IDH1-WT or IDH1-R132H. (b) Protein expression of HIF-1α in U87 cells transfected with IDH1-WT or IDH1-R132H. (c) Protein expression of HIF-1α in U251 cells transfected with IDH1-WT or IDH1-R132H.(d) Protein expression of HIF-1α in U87 and U251 cells transfected with shHIF-1α or shNC. (e) Proliferation of U87 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC. (f) Proliferation of U251 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC. (g) Apoptosis of U87 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC, in the presence or absence of TMZ. (h) Apoptosis of U251 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC, in the presence or absence of TMZ. ** P < 0.01

Figure 3: Increased expression of HIF-1α is involved in IDH1-R132H-induced inhibition of glioblastoma malignancy.(a) Protein expression of HIF-1αin U87 and U251 cells transfected with IDH1-WT or IDH1-R132H. (b) Protein expression of HIF-1α in U87 cells transfected with IDH1-WT or IDH1-R132H. (c) Protein expression of HIF-1α in U251 cells transfected with IDH1-WT or IDH1-R132H.(d) Protein expression of HIF-1α in U87 and U251 cells transfected with shHIF-1α or shNC. (e) Proliferation of U87 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC. (f) Proliferation of U251 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC. (g) Apoptosis of U87 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC, in the presence or absence of TMZ. (h) Apoptosis of U251 cells transfected with IDH1-WT or IDH1-R132H, and shHIF-1α or shNC, in the presence or absence of TMZ. ** <i>P</i> < 0.01