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Figure 4 : Results of Southern Blotting (SB) analysis. M = allele sizing ladder, (E) = size of the smaller allele after single digestion with EcoRI, (E/B) = size of the smaller allele after double digestion with EcoRI followed by BlnI. DNA for some individuals was limited hence only double digestion could be performed for them; the result for case 14 (clinically affected) could not be obtained as only a smear was seen. The individuals have been indicated as per case ID in Figure 1

Figure 4 : Results of Southern Blotting (SB) analysis. M = allele sizing ladder, (E) = size of the smaller allele after single digestion with EcoRI, (E/B) = size of the smaller allele after double digestion with EcoRI followed by BlnI. DNA for some individuals was limited hence only double digestion could be performed for them; the result for case 14 (clinically affected) could not be obtained as only a smear was seen. The individuals have been indicated as per case ID in Figure 1